BiologicalActivity
EnhancesCRISPR-Cas9-mediatedhomology-directedrepair(HDR)efficiencyinvitroupto19-fold.Inhibitsnonhomologousend-joining(NHEJ).
WehavediscoveredthatthecommerciallyavailableSCR7materialsuppliedbyothervendorsdoesnotmatchthechemicalstructurethatitisbeingsoldunder.ThroughdetailedchemicalanalysiscarriedoutbyourAnalyticalQualityControlexperts,webelievethatthecommerciallyavailableSCR7material,includingthatusedinrecentpublicationstoenhanceCRISPRefficiency,isinfactananalogofSCR7thatwehavenamed'SCR7pyrazine'.
ViewmoreinformationregardingthestructureofSCR7.
TechnicalData
| M.Wt | 332.38 |
| Formula | C18H12N4OS |
| Storage | Storeat+4°C |
| Purity | ≥98%(HPLC) |
| CASNumber | 14892-97-8 |
| PubChemID | 10688007 |
| InChIKey | GSRTWXVBHGOUBU-UHFFFAOYSA-N |
| Smiles | O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.44 | 100 | |
| ethanol | 6.69 | 20 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight332.38.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 3.01mL | 15.04mL | 30.09mL |
| 5mM | 0.6mL | 3.01mL | 6.02mL |
| 10mM | 0.3mL | 1.5mL | 3.01mL |
| 50mM | 0.06mL | 0.3mL | 0.6mL |
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