
TheProteinStABIlityComboKitMD1-103

Thermalshiftassaysprovideinformationontheeffectvariousenvironmentalfactorshaveonthethermaldenaturationofaprotein.Thermaldenaturationisrelatedtoproteinstabilityandthereforecrystallizability.ThemostcommonthermalshiftassayisThermoFluor®,whichmeasuresincreasingfluorescenceinducedbySYPRO®Orangebindingtotheexposedproteinhydrophobiccore.Buttherearemanyothersdependingontherequirementsofyoursample. Ourproteinstabilityscreensarenowavailableinonepackageatadiscountofmorethan20%comparedtobuyingthemseparately(orapproximately4kitsforthepriceof3). TheDurhamScreens TheDurhampHScreencontainsdeconvolutesbuffermoleculeandpHeffectsforaboradrangeofmolecule(28differentbuffers)andpH(4-11). TheDurhamSaltScreenusesmorethan30differentsalts,includingarangeoflanathanides.Itallowdiscriminationbetweendifferentanionsandcationsaswellasrevealingtheeffectsofionicstrength. ReadmoreaboutTheDurhamScreensin:Grøftehauge,MK,Hajizadeh,NR,Swann,MJandPohl,E.Protein-ligandinteractionsinvestigatedbythermalshiftassays(TSA)anddualpolarizationinterferometry(DPI).ActaCrystD71:36-44(2015). TheRUBICScreens TheRUBICBufferScreendeconstructsthebuffercomponentstoallowarationalinterpretationanddeconvolutionofpH,buffermoleculeandionicstrengtheffects. TheRUBICAdditiveScreenanalyzestheeffectonstabilityofvariouscommonadditivesincludingions,detergents,sugars,aminoacids,imidazole,cryoprotectantsandreducingagents.DevelopedbyEmilyCardewandEhmkePohlattheUniversityofDurham,thesekitsprovidegreaterdepthofanalysisoftheeffectofindividualbuffers,pHandsalts.TheDurhamscreenisdesignedtoworkwithNAMI,aGUI-basedprogramtoaidinterpretationofthermalshiftdata.
InventedbyStaphaneBoivinandRobMeijersattheSamplePerparationandCharacterisationfacilityatEMBLHamburg.TheRubicsscreenconsistsoftwokits:
TheRUBICScreensaredescribedin:BoivinS,KozakS,MeijersR.(2013)OptimizationofproteinpurificationandcharacterizationusingThermofluorscreens.ProteinExprPurif.91(2):192-206.
Reference:NewmanJ.(2004)Novelbuffersystemsformacromolecularcrystallization.ActaCrystallogrDBiolCrystallogr.60:610-2.
Storageconditions:
TheDurhampHScreenispresentedas96x0.5mLconditionsinadeep-wellblock.Theyarepreservativefreeanditisrecommendedthattheyarestoredat4°C,howevertheycanbeusedatroomtemperatureforscreenset-up.
RUBICBufferscreenisfreeofpreservatives.ShippingatRoomTemperature.Short-termStorageat4˚C.Itisrecommendedthatusersprefillplatesandstorethemat-20˚C.
ThermoFluorisaregisteredtrademarkofJohnsonandJohnson.SYPROisaregisteredtrademarkofLifeTechnologies.
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通过解答,教会我,谢谢
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么

