
CrystallophoreNo1(5x0.6mg)MD2-81

PolyvalanCrystallophoreNo1(patentpending)isaluminescentTerbiumcomplexthatisaneffectivenucleantandprovidessignificantisomorphousandanomalousphasingpower.Thisisthe5vialmultipackversionofthisproduct.ReadmoreabouthowCrystallophoreNo1workshere.
BREAKINGNEWS:ThefirstnovelstructuresolvedusingCrystallophoreNo1,BacteriophageT5distaltailprotein(PDBID:5MF2)hasjustbeenreleasedintheProteinDataBank.Couldyoursbethenext?Tellusaboutit!
FORMING
CrystallophoreNo1actsasanucleatingagentandimprovescrystalqualityofpre-existinghits.AsshownrightforT5phagewithandwithoutCrystallophoreNo1(topright).
FINDING
CrystallophoreNo1luminescesstronglyinthevisIBLeregionunderUVexcitationmakingdetectingcrystalsindropsandloopseasy.Seemiddleright.
PHASING
TerbiumisalanthanideandthereforehassignificantpotentialforuseasananomalousscattererandintrADItionalisomorphousreplacement.WhenCrystallophoreNo1hasbeenusedasanucleant,itisoftenfoundboundtotheproteinthusprovidinghighqualityphasingpower.
NEXTSTEPS
Tohelpyoubuildthestructureyoucandownloadthepdborcifformatco-ordinatefilesfortheCrystallophoreNo1complex.KindlyprovidedbyPolyvalan.
READALLABOUTIT
ThedevelopmentworkleadingtoCrystallophoreNo1hasnowbeenpublishedin:
Engilberge,S.,Riobé,F.,DiPietro,S.,Lassalle,L.,Coquelle,N.,Arnaud,C.-A.,Pitrat,D.,Mulatier,J.-C.,Madern,D.,Breyton,C.,Maury,O.andGirard,E.Crystallophore:aversatilelanthanidecomplexforproteincrystallographycombiningnucleatingeffect,phasingpropertiesandluminescence.ChemicalScience8:5909-5917(2017).(bottomright)P.horikoshiiProtease1electrondensityfromSADphasingusingtheTerbiumLIIIabsorptionedge.Crystalgrownin10mMandsubsequentlysoakedin100mMcrystallophoreNo1.
Eachvialccontains0.6mgofthecomplex,enoughtosetup696-wellplatesofyourtargetproteinincomplexwith10mMCrystallophoreNo1(recommendedconcentration).Shippedatroomtemperature.Thedrypowderwillkeepforupto1yearinasealedtubeatroomtemperatureindryconditions.SolutionsofCrystallophoreNo1canbekeptforupto2monthsinasealedtube.Solutionsofthecompoundmixedwithyourtargetproteinshouldbeusedimmediately.
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通过解答,教会我,谢谢
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么

