| ProductDetails | |
|---|---|
| Synonyms | MonophosphorylLipidA[MPLA]derivedfromE.coliR515(Re)LPS(biosynthetic) |
| ProductType | Chemical |
| Properties | |
| Source/HostChemicals | IsolatedandpurifiedfromE.colistrainR515. |
| PurityChemicals | ≥99.9%.NodetectableDNA,RNAandproteintraces. |
| Formulation | Liquid.Colourlessopaqueaqueoussolution. |
| Concentration | 1mg/ml(0.5mg/mlfor250µgsize)stABIlisedinsterile,double-distilledwater(ddWater),withoutanyadditives. |
| BIOLOGicalActivity | Optimalconcentrationisdependentuponcelltype,species,desiredactivationandanalysis:0.1-1.0μg/ml.DoesnotactivateanyTLRotherthanTLR4astestedupto50μg/mlinrelevantcellularsystems(macrophages). |
| Declaration | ManufacturedbyInnaxon. |
| OtherProductData | TLRpure™:•QualifiedPurity&Activity•HighpotencyTLR4-specificLigands•Ultrapure(nodetectableprotein,RNAandDNA)•TestedonTLR4KOmurinemacrophages•StandardisedAqueousSterileSolutions•Nopurificationorhazardoussolubilisation•Excellentlot-to-lotconsistency |
| ShippingandHandling | |
| Shipping | AMBIENT |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Donotfreeze. Ready-madesolutioniscellculture-grade.PreparedilutedMPLAworkingsolutionsinwaterjustpriortouse,keepsterile. Toyielda100μg/ml(1,000-100x)stocksolutionadd100μlofMPLAto900μlendotoxin-freeandsterileddWater(Cat.No.:IAX-900-002)(notPBS)andmixwell. |
| Use/Stability | Stableforatleast2yearsafterreceiptwhenstoredat+4°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
ActivationofcellsbyLPSismediatedbytheToll-likereceptor4(TLR4),amemberofthehighlyconservedproteinfamilyofTLRs,whicharespecialisedintherecognitionofmicrobialcomponents.Inmice,defectsinTLR4resultinLPSunresponsiveness.ForoptimalinteractionwithLPS,TLR4requiresassociationwithmyeloiddifferentiationprotein2(MD-2).AccordingtocurrentconsensusactivationofTLR4isprecededbythetransferofLPStomembrane-bound(m)orsoluble(s)CD14byLPS-bindingprotein(LBP).ThismechanismisbelievedtobegenerallytrueforLPSsignaling.Re-formLPSandlipidA,butnotS-formLPS,arecapableofinducingTNF-αresponsesalsointheabsenceofCD14.LPS,synthesizedbymostwild-type(WT)Gram-negativebacteria(S-formLPS),consistsofthreeregions,theO-polysaccharidechain,whichismadeupofrepeatingoligosaccharideunits,thecoreoligosaccharideandthelipidA,whichharborstheendotoxicactivityoftheentiremolecule.R-formLPSsynthesizedbytheso-calledrough(R)mutantsofGram-negativebacterialackstheO-specificchain.FurThermore,thecore-oligosaccharidemaybepresentindifferentdegreesofcompletion,dependingontheclass(RatoRe)towhichthemutantbelongs.MonophosphorylLipidA(MPLA)representsadetoxifiedderivativeofLipidAandconstitutesanimportantadjuvantinprophylacticandtherapeuticvaccines.
- AnewmethodfortheextractionofRlipopolysaccharides:C.Galanos,etal.;Eur.J.Biochem.9,245(1969)
- Preparationandpropertiesofantiseraagainstthelipid-Acomponentofbacteriallipopolysaccharides:C.Galanos,etal.;Eur.J.Biochem.24,116(1971)
- EndotoxicpropertiesofchemicallysynthesizedlipidApartstructures.ComparisonofsyntheticlipidAprecursorandsyntheticanalogueswithbiosyntheticlipidAprecursorandfreelipidA:C.Galanos,etal.;Eur.J.Biochem.140,221(1984)
- Lipopolysaccharides:structuralprinciplesandbiologicactivities:O.Luederitz,etal.;Rev.Infect.Dis.6,428(1984)
- SyntheticandnaturalEscherichiacolifreelipidAexpressidenticalendotoxicactivities:C.Galanos,etal.;Eur.J.Biochem.148,1(1985)
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请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
通过解答，教会我，谢谢
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
