TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-1β/IL-6/TNF-αFluoroSpotkitisperfectforuserswhowishtohaveaconvenientassaywithminimizedassayvariABIlity.Itenablesenumerationofcellssecretingone,twoorthreeanalytes.Thekitincludeslowfluorescentplatespre-coatedwithmonoclonalcaptureantibodies,monoclonaldetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincludedaspositivecontrolstimulus.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforexcitation490nm/emission510nm(LED490,FITC)for490-reagentdetection,excitation550nm/emission570nm(LED550,Cy3)for550-reagentdetectionandexcitation640nm/emission660nm(LED640,Cy5)for640-reagentdetection.Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.
AnalyteInformation
IL-1βInterleukin-1ß(IL-1ßorIL-1F2,alsoknownasInterleukin-1beta,IL-1beta,IL1bandInterleukin-1beta)isaproinflammatorycytokineandinducerofacutephaseresponses.IL-1ßisproducedprimarilybymonocytes,macrophages,anddendriticcellsafterinductionbymicrobes.
IL-6Interleukin6(IL-6,IL6)isapleiotropiccytokinethatisproducedbymanydifferentcelltypesandplaysaroleinawiderangeoffunctions,suchasimmuneresponses,acute-phasereactions,andhematopoiesis.AmongotherthingsitaugmentsantibodyproductionfromactivatedBcellsinvitro.
TNF-αTumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.| Application: |
|
|---|---|
| Reactivity: |
|
| Format: | FluoroSpotPLUS |
| Documentation: | Datasheet/Protocol |
| Contents: |
|
| Size: | 10plates |
| Otherformats: | ClicktoviewallHumanIL-1β,IL-6,TNF-αproducts |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
尤其是获得SCI文献引用的产品,
绝对ok的
请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!

