I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.
Mouse IgG2a ELISpotBASIC enables enumeration of B cells secreting total IgG2a as well as antigen-specific IgG2a. The IgG2a allele is expressed in e.g., Balb/c mice. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP and polyclonal activators.
With this kit, detection of antigen-specific IgG2a secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody, which is available in the web shop. See Related products.
For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).
IgG2a ELISpotBASIC is also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates.See Related products.
Analyte Information
IgG2aIgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3.The IgG2a allele is expressed in e.g. Balb/c mice.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Documentation: | Datasheet/ProtocolSDS |
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| Size: | for 4 plates |
| Other formats: | Click to view all Mouse IgG2a products |
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我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
尤其是获得SCI文献引用的产品,
绝对ok的
请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!

