Most mammalian cells carry two copies of most genes. CRISPR/Cas9 gene editing can introduce insertion or deletion mutations (indels) on either one or both copies of a given gene. The Guide-it Genotype Confirmation Kit provides a simple method for determining if a given clone has mutations in one copy (monoallelic), both copies (biallelic), or is unchanged (wild type). The protocol involves amplification of the target site from cells treated with Cas9 and a single guide RNA (sgRNA).
Most mammalian cells carry two copies of most genes. CRISPR/Cas9 gene editing can introduce insertion or deletion mutations (indels) on either one or both copies of a given gene. The Guide-it Genotype Confirmation Kit provides a simple method for determining if a given clone has mutations in one copy (monoallelic), both copies (biallelic), or is unchanged (wild type). The protocol involves amplification of the target site from cells treated with Cas9 and a single guide RNA (sgRNA). The resulting amplicon is used in an in vitro cleavage reaction with recombinant Cas9 and the same sgRNA. The genotype can be determined by resolving the cleavage products on an agarose gel.
Using this kit after gene editing expedites the process of screening a large number of clones for those with the desired genotype. Each kit contains sufficient reagents for 100 extractions, amplifications, and cleavage reactions.
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我想用酶联免疫斑点（ELISPOT）技术测定NK细胞活性，但不知道哪里能买到试剂盒，特向大家求助，谢谢。
尤其是获得SCI文献引用的产品，
绝对ok的
请问一般的实验室可以进行ELIspot吗？（我可以买到现成的试剂盒，资金这个应该没有问题。）ELIspot的难点在哪里？多谢大虾们的指点！这个对我很重要，多谢！！！
