
Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1 Kappa |
Purification : | Protein G Chromatography |
Content : | 0.2 mg/ml in Tris buffer containing 0.05% Azide |
Storage condition : | Store the antibody at 4°C, stable for 6 months. |
Gene : | TLR6 |
Gene ID : | 10333 |
Uniprot ID : | Q9Y2C9 |
Alternative Name : | TLR6 |
Immunogen Information : | A partial length recombinant TLR6 protein (amino acids 280-512) was used as the immunogen for this antibody. |
TLR6 (Toll-Like Receptors 6) is a member of the TLR (Toll-like receptor) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLR6 forms heterodimers with TLR1 and TLR2, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens. TLR2 in co-operation with TLR6 trigger inflammatory responses via activation of NF-kappaB, which regulates the transcription of inflammatory molecules. TLR6 has emerged as the most potent activator for ZNPs (Zinc Oxide Nanoparticles)-induced inflammatory responses. Upon silencing, TLR6 significantly inhibited the pro-inflammatory cytokine levels, reactive nitrogen species generation and inducible nitric oxide synthase expression.
FACS analysis:0.5-1 µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cell membrane, Cytoplasmic vesicle, Membrane raft, Golgi apparatus |
Tissue Specificity: | Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells. |
BioGrid: | 115616.3 interactions. |
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请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。

