Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal cells via a simple extraction method using animal cells cultured in 96-well (or other size well) plates. Direct RNA template purification from animal cells for use in real-time PCR takes approximately 10 minutes.
Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal cells via a simple extraction method using animal cells cultured in 96-well (or other size well) plates. Direct RNA template purification from animal cells for use in real-time PCR takes approximately 10 minutes.
When used with a one-step real-time RT-PCR kit, theCellAmp Direct RNA Prep Kit (Real Time) & Protein Analysis allows gene expression analysis to be completed in approximately two hours. When combined with a reverse transcription reagent such as the PrimeScript RT Reagent Kit (Perfect Real Time), cDNA templates for real-time PCR can be synthesized in approximately 30minutes, enabling efficient high-throughput analyses. Direct RNA template purification can be accomplished using small batches of animal cells, a useful feature for gene expression profiling of precious or rare samples. The kit is also useful for studies of genes that are expressed at very low levels. Genomic DNA can be easily and efficiently removed using this kit. Finally, RNA template samples prepared withCellAmp Direct RNA Prep Kit (Real Time) & Protein Analysis are compatible with intercalating green dye and 5" nuclease probe-based assays for real-time PCR (qPCR), and thus can serve a wide variety of experimental applications.
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我想用酶联免疫斑点（ELISPOT）技术测定NK细胞活性，但不知道哪里能买到试剂盒，特向大家求助，谢谢。
尤其是获得SCI文献引用的产品，
绝对ok的
请问一般的实验室可以进行ELIspot吗？（我可以买到现成的试剂盒，资金这个应该没有问题。）ELIspot的难点在哪里？多谢大虾们的指点！这个对我很重要，多谢！！！
