TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-1β/GM-CSFFluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincluded.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
GM-CSF
Granulocytemacrophage-colonystimulatingfactor(GM-CSF)canbeproducedbyseveralcelltypesamongothersactivatedTcells,macrophages,endothelialcellsandstromalcells.GM-CSFstimulatesthesurvivalandfunctionalactivitiesofneutrophils,eosinophils,andmonocyte-macrophages.ItalsostimulatesthedifferentiationandproliferationofhematologicalProgenitors.
IL-1β
Interleukin-1ß(IL-1ßorIL-1F2,alsoknownasInterleukin-1beta,IL-1beta,IL1bandInterleukin-1beta)isaproinflammatorycytokineandinducerofacutephaseresponses.IL-1ßisproducedprimarilybymonocytes,macrophages,anddendriticcellsafterinductionbymicrobes.
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| Size: | 10plates |
| Otherformats: | ClicktoviewallHumanGM-CSF,IL-1βproducts |
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我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
尤其是获得SCI文献引用的产品,
绝对ok的
请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!

