
TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-1β/IL-6FluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincluded.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
IL-1β
Interleukin-1ß(IL-1ßorIL-1F2,alsoknownasInterleukin-1beta,IL-1beta,IL1bandInterleukin-1beta)isaproinflammatorycytokineandinducerofacutephaseresponses.IL-1ßisproducedprimarilybymonocytes,macrophages,anddendriticcellsafterinductionbymicrobes.
IL-6
Interleukin6(IL-6,IL6)isapleiotropiccytokinethatisproducedbymanydifferentcelltypesandplaysaroleinawiderangeoffunctions,suchasimmuneresponses,acute-phasereactions,andhematopoiesis.AmongotherthingsitaugmentsantibodyproductionfromactivatedBcellsinvitro.
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Size: | 2plates |
Otherformats: | ClicktoviewallHumanIL-1β,IL-6products |
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尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。

