TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-6/TNF-αFluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincluded.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
IL-6
Interleukin6(IL-6,IL6)isapleiotropiccytokinethatisproducedbymanydifferentcelltypesandplaysaroleinawiderangeoffunctions,suchasimmuneresponses,acute-phasereactions,andhematopoiesis.AmongotherthingsitaugmentsantibodyproductionfromactivatedBcellsinvitro.
TNF-α
Tumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.
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| Size: | 2plates |
| Otherformats: | ClicktoviewallHumanIL-6,TNF-αproducts |
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我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
尤其是获得SCI文献引用的产品,
绝对ok的
请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!

