
TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanTNF-α/IL-12/-23(p40)FluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophoresandafluorescenceenhancer.LPSisincluded.
Theassayisrecommendedfortheanalysisofmacrophages,monocytesanddendriticcells.ThesekitshavenotbeenevaluatedforT-cells.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
IL-12/-23(p40)
Interleukin-12(IL-12,IL12)is,whenBIOLOGicallyactive,aheterodimer(p70)consistingoftwocovalentlylinkedsubunits,p35andp40.IL-12isproducedbyantigen-presentingcells,specificallydendriticcellsandmacrophages.IL-12isaproinflammatorycytokinethatpromotesTh1-typeresponsesbyinducingIFN-γproductionandenhancingproliferationandcytotoxicityofNKandTcells.Interleukin-23(IL-23)isadisulfide-linkedheterodimerwhichconsistsofauniquep19subunitandthep40subunitofIL-12.IL-23isaproinflammatorycytokine,whichisproducedbymacrophagesanddendriticcellsuponinfectionswithcertainpathogens.TheIL-23receptorisexpressedonTh17cellsandIL-23isresponsIBLeforTh17expansionandstABIlization.SincebothIL-12andIL-23heterodimerscomprisethep40subunit,captureimmunoassaysbasedonantibodiestop40willdetectbothcytokines.Suchassayscanalsodetectp40monomersanddimers.
TNF-α
Tumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.
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Size: | 10plates |
Otherformats: | ClicktoviewallHumanIL-12/-23(p40),TNF-αproducts |
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尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。

