
TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes,whichisamajoradvantage.ThehumanIL-17A/IL-22FluoroSpotkitenablesconvenientenumerationofcellssecretingeitheroneorbothanalytes.Thekitcontainslowfluorescentplates,matchedquantitiesofmonoclonalcaptureanddetectionantibodies,secondaryreagentsconjugatedtofluorophores,afluorescenceenhancerandananti-CD28monoclonalantibodyforco-stimulation.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.
HumanIL-17A/IL-22FluoroSpotkitsareavailablein2or10-plateformat.
Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.Doublesecretingcellsareidentifiedbyacomputerizedoverlay.
AnalyteInformation
IL-17A
Interleukin-17A(IL-17A,IL17A)isapotentproinflammatorycytokineproducedbyactivatedTh17(T-helper17)cellsandcertaincellsbelongingtotheinnateimmunesystem.Inmice,IL-17hasbeenshowntoalsobeproducedbyactivatedCD8+TcellsandγδTcells.Th17cellsplayanimportantroleinautoimmunediseasesandprotectionagainstbacteriaandfungi.IL-17Aactsonabroadrangeofcelltypestoinduceexpressionofcytokines,chemokinesandmetalloproteinases.Asaresult,secretionofIL-17Apromotesinflammatoryresponses,whichleadstotherecruitmentofneutrophils,enhancementofantibodyproductionandactivationofTcells.IncreasedexpressionofIL-17Aisseeninautoimmunediseasessuchasmultiplesclerosisandrheumatoidarthritis.Itisalsoassociatedwithasthma,psoriasis,cancerandtransplantrejection.
IL-22
ThecytokineInterleukin-22(IL-22,IL22)ismainlyproducedbyactivatedCD4+T-helper17(Th17)andT-helper22(Th22)cells.TheIL-22receptorisexpressedonnon-immunecells,particularlyepithelialcellsandkeratinocytes,andIL-22promotesinnateimmuneresponsesversusbacterialinfectionsinthesetargetcells.Inaddition,Th22cellshavebeenshowntoplayaroleinthepathophysiologyofmanyhumanskindiseases.
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Size: | 10plates |
Otherformats: | ClicktoviewallHumanIL-17A,IL-22products |
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尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。

