Liposome formulations can be broadly divided into cationic, anionic and neutral subtypes. Charged liposomes are used in many different types of studies such as, blood complement studies, gene transfer studies and other biomedical applications to name a few. Cellsome® liposomes made from cationic PEGylated lipids provide certain benefits. Cationic liposomes interact with negatively charged glycoproteins (i.e. mucins) on the cell surface, thereby increasing the affinity and residence time with the target cells, resulting in increased drug delivery. Conventional liposomal formulations reduced the toxicity of compounds in vivo, through modifying pharmacokinetics and biodistribution to enhance drug delivery to diseased tissue in comparison to free drug. However, the delivery system was prone to rapid elimination from the bloodstream, therefore limiting its therapeutic efficacy. To improve liposome stability and enhance their circulation times in the blood, a sterically-stabilized, hydrophilic polymer, polyethylene glycol (PEG), has been shown to be the optimal choice for obtaining sterically-stabilized liposomes. Using a Cationic Liposome with PEG will protect the liposomes from circulating proteins, improving their plasma clearance and enhancing their therapeutic effects.
The two products provided that are made from Cationic PEGylated Lipids provided here consist of either DSPC:Chol:DSTAP:DSPE-PEG(2000) (60:30:5:5 molar ratio) or DOPC:Chol:DOTAP:DOPE-PEG(2000) (60:30:5:5 molar ratio).
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我想用酶联免疫斑点（ELISPOT）技术测定NK细胞活性，但不知道哪里能买到试剂盒，特向大家求助，谢谢。
尤其是获得SCI文献引用的产品，
绝对ok的
请问一般的实验室可以进行ELIspot吗？（我可以买到现成的试剂盒，资金这个应该没有问题。）ELIspot的难点在哪里？多谢大虾们的指点！这个对我很重要，多谢！！！
