WesternBlottingHRPSubstrate
lumigenECLUltra(TMA-6)isaproprietaryacridanbasedchemiluminescentsubstrateforultra-sensitivedetectionofWesternblottedandELISAcapturedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.
- Veryrapidonsetofchemiluminescence- imagescanberecordedinseconds
- Highestsensitivity -midtolowfemtogram
- Reducedantibodyusage - morethan10-foldreductionintheusageofexpensiveantibodyconjugates
- Temperatureinsensitive - analyticalresultsareinsensitivetotemperaturesfrom22°C - 35°Creducingtheneedforprecisetemperaturecontrol
- Sustainedsignalduration - overseveralhoursallowsconvenientimaging
StableWorkingSolution
SimilartoLumigenECLExtra,LumigenECLUltrahasexcellentstABIlity.Theworkingsolutionisstableatroomtemperatureforatleast oneweekandat2-8°Cforatleast onemonth.Withthisstability,theworkingsolutioncanbepreparedandstoredforlateruse.
RapidPeakIntensity
ReactionofthesubstratewithHRPenzymegeneratesrapidluminescenceofthehighestintensitywithinseconds.Peakintensityoflightemissionoccursat440nm.
ProductSpecifications
ebiomall.com
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耗材:滤纸、PVDF膜或NC膜、乳胶手套等
仪器:电泳仪、电转槽、摇床、制冰机(电转时需冰浴)等
转膜后就要区分与胶接触的一面与另一面了。话说楼主好奇的话可以跑蛋白的试试看哈。比如目的蛋白的胶用光滑面贴着胶,内参蛋白用粗糙面贴着胶一起转膜试试转膜结果看看呢。
当然做都是可以做,需要带上阴性和阳性的对照组,以区别出是抗体制备的问题还是抗原决定簇被破坏的问题。