Accuris High Fidelity DNA Polymerase
· 50X higher fidelity than Taq DNA polymerase.
· Works with crude DNA samples.
· Ideal for cloning, mutagenesis and microarrays.
· Optimized buffer system with unique PCR enhancers.
For applications requiring highly accurate amplification, choose Accuris High Fidelity DNA Polymerase. Modified for better sensitivity and higher activity for more reproducible performance, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples. A 3-5 proofreading exonuclease activity and an error rate of 4.55x10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

