Overview:
PAK6 is a Ser/Thr protein kinase that is a member of the PAK (p21-activated kinase) family that are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 is highly expressed in the testis and prostate tissues and interacts with the androgen receptor (AR). In response to androgen, PAK6 cotranslocate into the nucleus with AR (1). PAK6 is weakly expressed in normal prostate epithelium but the expression is increased in primary and metastatic prostate cancer cells and is further increased in tumors that relapse after androgen deprivation therapy (2).
Gene Aliases:
PAK5
Genbank Number:
NM_020168
References:
1.Yang F, et al: Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6. J. Biol. Chem. 276: 15345-15353, 2001. 2.Kaur R, et al: Increased PAK6 expression in prostate cancer and identification of PAK6 associated proteins. Prostate. 2008 Oct 1;68(14):1510-6.
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

