Overview:
TIE2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggests a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE2 receptor tyrosine kinase (1). SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE2 function. TIE2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE2 is also overexpressed in areas of active angiogenesis in normal tissues (2).
Gene Aliases:
TIE-2, TEK, VMCM, VMCM1, CD202B
Genbank Number:
NM_000459
References:
1. Woolf, A S. et al: Angiopoietin growth factors and Tie receptor tyrosine kinases in renal vascular development. Pediatr Nephrol. 2001 Feb;16(2):177-84. 2. Peters, K G. et al: Functional significance of Tie2 signaling in the adult vasculature. Recent Prog Horm Res. 2004;59:51-71.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

