Overview:
TPM3 is an actin-binding protein that mediates myosin–actin response to calcium ions in skeletal muscles. PDGFRB is a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family.PDGFRB is located at chromosome 5q31-33, and has been reported to have at least 18 fusion partners. It was uncovered that the TPM3 gene at 1q21 as a PDGFRB partner in chronic eosinophilic leukemia (CEL).
Gene Aliases:
TPM3: CAPM1; CFTD; hscp30; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TM5; TPMsk3PDGFRB:JTK12, PDGFR, CD140B, PDGFR1, PDGF-R-beta
Genbank Number:
NM_152263NM_002609
References:
1. Rosati R, et al. TPM3/PDGFRB fusion transcript and its reciprocal in chronic eosinophilic leukemia. Leukemia. 20(9):1623-4, 2006.2. Li Z, Yang R, Zhao J, Yuan R, Lu Q, Li Q, Tse W. Molecular diagnosis andtargeted therapy of a pediatric chronic eosinophilic leukemia patient carrying TPM3-PDGFRB fusion. Pediatr Blood Cancer. 56(3):463-6. 2011.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

