Overview:
TPM3 is an actin-binding protein that mediates myosin–actin response to calcium ions in skeletal muscles. PDGFRB is a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family.PDGFRB is located at chromosome 5q31-33, and has been reported to have at least 18 fusion partners. It was uncovered that the TPM3 gene at 1q21 as a PDGFRB partner in chronic eosinophilic leukemia (CEL).
Gene Aliases:
TPM3: CAPM1; CFTD; hscp30; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TM5; TPMsk3PDGFRB:JTK12, PDGFR, CD140B, PDGFR1, PDGF-R-beta
Genbank Number:
NM_152263NM_002609
References:
1. Rosati R, et al. TPM3/PDGFRB fusion transcript and its reciprocal in chronic eosinophilic leukemia. Leukemia. 20(9):1623-4, 2006.2. Li Z, Yang R, Zhao J, Yuan R, Lu Q, Li Q, Tse W. Molecular diagnosis andtargeted therapy of a pediatric chronic eosinophilic leukemia patient carrying TPM3-PDGFRB fusion. Pediatr Blood Cancer. 56(3):463-6. 2011.
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

