Overview:
TPM3 is a member of the tropomyosin family of actin-binding proteins which are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in TPM3 gene result in autosomal dominant nemaline myopathy and other muscle disorders (1). TPM3 gene locus is involved in translocations with other loci including c-ros oncogene 1 (ROS1) which result in the formation of the TPM3-ROS1 fusion protein that act as an oncogene. TPM3-ROS1 fusion gene product has been detected in lung adenocarcinoma is higher in lung adenocarcinoma with wild-type EGFR (2).
Gene Aliases:
TPM3: CAPM1; CFTD; hscp30; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TM5; TPMsk3; TRKROS1: c-ros-1; MCF3; ROS
Genbank Number:
NM_152263NM_002944
References:
1. Lawlor, M W. et al: Mutations of tropomyosin 3 (TPM3) are common and associated with type 1 myofiber hypotrophy in ongenital fiber type disproportion. Hum Mutat. 2010 Feb;31(2):176-83.2. Zhao C. et al: Detecting ALK, ROS1 and RET Fusion Genes in Cell Block Samples. Transl Oncol. 2014 Jun 17;7 (3):363-7.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

