Overview:
TNF-alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily and it is secreted predominantly by monocytes/macrophages that have effects on lipid metabolism, coagulation, insulin resistance, and endothelial function (1).TNF-alpha is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation and implicated in a variety of diseases. TNF-alpha also participates in the homeostatic activity-dependent regulation of synaptic connectivity and play roles in synaptic plasticity and modulating responses to neural injury (2).
Gene Aliases:
TNF, TNFa, DIF, TNFA, TNFSF2
Genbank Number:
P01375
References:
1. Shirai, T.et.al: Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. Nature 313: 803-806, 1985.2. Stellwagen D. et al: Synaptic scaling mediated by glial TNF-alpha. Nature. 2006 Apr 20; 440(7087):1054-9.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

