Overview:
TNF-beta is a member of the tumor necrosis factor cytokine family that are primarily expressed on lymphocytes that control lipid metabolism. TNF-beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF-beta acts as a novel therapeutic route for the treatment of dyslipidemia (1). The presence of TNF-beta accelerates the initiation of immune responses and protects the host from larger viral challenges (2).
Genbank Number:
P09225
References:
1. Lo, J. C. et.al: Lymphotoxin beta receptor-dependent control of lipid homeostasis. Science 316: 285-288, 2007. 2. Lund, F. E. et.al: Lymphotoxin-alpha-deficient mice make delayed, but effective, T and B cell responses to influenza. J. Immun. 169: 5236-5243, 2002.
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

