Overview:
hGH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control and it is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. hGH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland (1).The systemic inhibition of GH or IGF1, or both, may have therapeutic potential in preventing some forms of retinopathy (2).
Gene Aliases:
GH; GHN; GH-N; hGH-N; IGHD1B
Genbank Number:
NP_000506
References:
1. Niall, H. D. et.al: Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971.2. Smith, L. E. H. et.al: Essential role of growth hormone in ischemia-induced retinal neovascularization. Science 276: 1706-1709, 1997.
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

