Overview:
There are two functionally comparable forms of IL1: IL1A and IL1B, that are encoded by two different genes. IL1B is the predominant form in humans while, it is IL1A in mice. Both forms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. Latter include but not limited to, stimulation of T-helper cells, promotion, proliferation and synthesis of immunoglobulins and the activation of fibroblasts and NK cells. Unlike IL1B, the IL1A precursor does not require processing before it can bind to the receptor. Monocytes are the main source of secreted IL1B while human keratinocytes are known to express large amounts of IL1A. Recombinant Human IL1A is a non-glycosylated polypeptide of 18 kDa.
Gene Aliases:
Hematopoietin-1, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, IL1F1, Recombinant Human Interleukin-1 Alpha (IL1A)
Genbank Number:
P01583
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

