Overview:
Irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) IC50 < 2="">1,2. Tranylcypromine treatment of P19 embryonal carcinoma cells resulted in global increase of H3K4 methylation along with transcriptional derepression of two BHC110 target genes, Egr1 and Oct4. When combined with CHIR99021, induces the generation of human-induced pluripotent stem cells in the absence of exogenous Sox23.Reduces proliferation, cell cycle progression and invasiveness of endometriotic stromal cells4. Upregulates CD86 expression in THP1 cells5. Cell permeable and active in vivo.
References:
1. NG Lee et al. Chem. Biol. 2006 13:5632. DM Schmidt and DG McCafferty Biochemistry 2007 46:44083. W Li et al.Stem Cells 2009 27:29924. DDing et al. Fertil. Steril. 2014 101:7405. JT Lynch et al. Anal. Biochem. 2013 442:104
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

