Overview:
Irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) IC50 < 2="">1,2. Tranylcypromine treatment of P19 embryonal carcinoma cells resulted in global increase of H3K4 methylation along with transcriptional derepression of two BHC110 target genes, Egr1 and Oct4. When combined with CHIR99021, induces the generation of human-induced pluripotent stem cells in the absence of exogenous Sox23.Reduces proliferation, cell cycle progression and invasiveness of endometriotic stromal cells4. Upregulates CD86 expression in THP1 cells5. Cell permeable and active in vivo.
References:
1. NG Lee et al. Chem. Biol. 2006 13:5632. DM Schmidt and DG McCafferty Biochemistry 2007 46:44083. W Li et al.Stem Cells 2009 27:29924. DDing et al. Fertil. Steril. 2014 101:7405. JT Lynch et al. Anal. Biochem. 2013 442:104
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

