Human CD21 is a receptor for complement fragments C3d, C3dg or ic3D (1, 5) and also EBV (4). CD21 is also a ligand for CD23 and plays a role in IgE synthesis (3).
Isotype: Murine IgG1
Immunogen: Human HFB-1 cell line
Specificity: Antibody BU33 recognizes the CD21 molecule of about 145 kd (1). BU33 has been shown to bind to Equine B cells, suggesting cross reactivity (7).
Functional Application: Antibody BU33 inhibits the CD21 - CD23 interaction (2, 6, 8).
References:
1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 516-523.
2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 535-538.
3. J.Y. Bonnefoy, et al, (1995) Intl Archiv Allergy Immunol 107: 40-42.
4. A.D. Beaulieu, et al, (1995) Blood 86: 2789-2798.
5. H. Molina, et al, (1995) J Immunol 154: 5426-5435.
6. S Henchoz, J Y Bonnefoy, et al. (1994) Immunology 81(2): 285-290.
7. S. Mayall, Hamblin A.S., et al. (2001) J Comp Pathol 124(1): 83-87.
8. S Henchoz, J Y Bonnefoy, et al. (1996) Immunolgy 88: 35-39.
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

