| AEG 3482inhibitor of Jun kinase (JNK)-dependent apoptosis |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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- Purity = 99.57%
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Chemical structure
| Cell experiment [1]: | |
Cell lines | PC12 neuronal cells |
Preparation method | The solubility of this compound in DMSO is > 14 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 10, 20 and 40 µM, 18 h |
Applications | AEG 3482 is a potent antiapoptotic compound. In PC12 neuronal cells AEG 3482 inhibited proapoptotic JNK activity and blocked p75NTR- and NRAGE-induced apoptosis. AEG 3482 inhibited the JNK pathway through increasing HSP70 expression. |
References: [1]. Salehi A H, Morris S J, Ho W C, et al. AEG3482 is an antiapoptotic compound that inhibits Jun kinase activity and cell death through induced expression of heat shock protein 70[J]. Chemistry & biology, 2006, 13(2): 213-223. | |
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| Cas No. | 63735-71-7 | SDF | Download SDF |
| Chemical Name | 6-phenylimidazo[2,1-b][1,3,4]thiadiazole-2-sulfonamide | ||
| Canonical SMILES | NS(C1=NN(C(S1)=N2)C=C2C3=CC=CC=C3)(=O)=O | ||
| Formula | C10H8N4O2S2 | M.Wt | 280.33 |
| Solubility | ≥14mg/mL in DMSO | Storage | Store at RT |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
AEG3482 is a small-molecule inhibitor of Jun kinase (JNK)-dependent apoptosis. [1,2]
Jun kinases, also called Jun N-terminal kinase stress-activated protein kinases. Activation of the JNK signaling pathway is amajor event in neuronal apoptosis. This is proved by pathological specimens from Alzheimer’s disease, as well as samples from mouse models of Alzheimer’s and Huntington’s disease, reveal activated JNK. [2]
AEG3482 blocks apoptosis induced by the p75 neurotrophin receptor (p75NTR) or NRAGE, which is p75NTR’s cytosolic interactor,. AEG3482 inhibits proapoptotic JNK activity. AEG3482 induces production of heat shock protein 70 (HSP70) which is an endogenous inhibitor of JNK. HSP70 accumulation is required for the AEG3482-induced JNK blockade. [1]
As described, activation of C-jun N-terminal kinase (JNK) signaling is associated with neuronal cell death. AEG3482 interacts with heat shock protein 90 leading to inhibition of JNK and blockade of neuronal apoptosis. So AEG3482 can serve as useful tools for basic research, and it may have therapeutic potential for the treatment of acute and chronic neurological disorders. In the neuropathologicalstate, the chaperone-mediated stress response may be primed for response to Hsp90 inhibitors; and AEG3482, may be selective neuroprotective agents.[1,2]
References:[1]Salehi AH, Morris SJ, Ho WC, etal., AEG3482 is an antiapoptotic compound that inhibits Jun kinase activity and cell death through induced expression of heat shock protein 70. Chem Biol. 2006 Feb;13(2):213-23.[2] Gallo KA. Targeting HSP90 to halt neurodegeneration. Chem Biol. 2006 Feb;13(2):115-6.
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我在做5RACE,选用TaKaRa公司的RNA反转录酶（RNaseH-），不知道这个酶好不好，有什么需要注意的。
没有序列识别的功能，他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应，需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接，也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶（Reversetranscripatase）是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA（cDNA）的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。
