| 9,12-Octadecadiynoic AcidCOX and lipoxygenase inhibitor |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
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| Cas No. | 2012-14-8 | SDF | Download SDF |
| Synonyms | Ro 3-1314 | ||
| Chemical Name | 9,12-octadecadiynoic acid | ||
| Canonical SMILES | CCCCCCCCCCCCCCCCCC(=O)O | ||
| Formula | C18H28O2 | M.Wt | 276.4 |
| Solubility | ≤10mg/ml in ethanol;10mg/ml in DMSO;10mg/ml in methanol;10mg/ml in acetone;10mg/ml in acetonitrile | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Ki: 0.6 μM for ram seminal vesicle COX
9,12-Octadecadiynoic acid is a COX and lipoxygenase inhibitor.
Cyclooxygenase (COX) is an enzyme responsible for the formation of prostanoids, such as thromboxane and prostaglandins. Pharmaceutical inhibition of COX can lead to relief from the symptoms of inflammation and pain. Nonsteroidal anti-inflammatory drugs, including aspirin and ibuprofen, display their effects via inhibition of COX.
In vitro: 9,12-Octadecadiynoic acid was identified as an inhibitor of both COX and lipoxygenase, which could inhibit ram seminal vesicle COX. 9,12-Octadecadiynoic acid was found to be a more effective inhibitor of COX-1 than of 15-LO when used at a concentration of 48 μM [1,2]. In another study, fatty acids from natural sources including 9,12-octadecadiynoic acid were isolated and assayed for their effect on the bioconversion of arachidonic acid into prostaglandin E2. Two very active cyclooxygenase inhibitors were discovered, namely jacarandic acid and the synthetic 8Z, 10E, 12E-octadecatrienoic acid. Earlier described potent inhibitors showed the following IC50 values: 1.3 μM for indomethacin; 1.3 μM for 9,12-octadecadiynoic acid, 2.7 μM for 8Z, 12E, 14Z-eicosatrienoic acid; 4.4 μM for 5,8,11,14-eicosatetraynoic acid [3].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
References:[1] Vanderhoek, J.Y. and Lands, W.E.M. Acetylenic inhibitors of sheep vesicular gland oxygenase. Biochimica et Biophysica Acta 296, 374-381 (1973).[2] Downing, D.T.,Barve, J.A.,Gunstone, F.D., et al. Structural requirements of acetylenic fatty acids for inhibition of soybean lipoxygenase and prostaglandin synthase. Biochimica et Biophysica Acta 280, 343-347 (1972).[3] Nugteren DH, Christ-Hazelhof E. Naturally occurring conjugated octadecatrienoic acids are strong inhibitors of prostaglandin biosynthesis. Prostaglandins. 1987 Mar;33(3):403-17.
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多谢……
各位大侠：小弟最近做个长片段的CDNA与T载体的连接，片段大小6.4K，载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621)，CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板，操作步骤严格按照说明做的，也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段，与载体连接，结果筛选到的几个克隆，插入的片段仅有2KB左右，奈何？可最近几次再用此反转录试剂盒却不能得到所需片段。在此，小弟想问问大家，你们是否遇到同样的问题，是如何解决的？所用的试剂（载体和酶的选择）和方法是什么，可以告诉后来者，共同进步吧。
