| nor-NOHA (acetate)potent, reversible inhibitor of rat liver arginase |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
- View current batch:
- Purity ≥ 97.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
nor-NOHA (acetate) Dilution Calculator
calculate
nor-NOHA (acetate) Molarity Calculator
calculate
| Cas No. | 1140844-63-8 | SDF | Download SDF |
| Synonyms | Nω-hydroxy-nor-Arginine | ||
| Chemical Name | 2S-amino-4-[[(hydroxyamino)iminomethyl]amino]-butanoic acid, diacetate | ||
| Canonical SMILES | N/C(N([H])CC[C@H](N)C(O)=O)=NO.CC(O)=O.CC(O)=O | ||
| Formula | C5H12N4O3 • 2C2H4O2 | M.Wt | 296.3 |
| Solubility | ≤5mg/ml in DMSO;1mg/ml in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A lyophilized powder | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Ki: 0.5 μM for rat liver arginase
nor-NOHA is a potent and reversible inhibitor of rat liver arginase.
L-Arginine is a common substrate for both nitric oxide synthase (NOS) and arginase in the cell. NOS an catalyze the oxidation of arginine to citrulline and NO. Arginase, on the other hand, catalyzes the hydrolysis of arginine into urea and L-ornithine.
In vitro: Previous study found that nor-NOHA could inhibit the proliferation and induce the apoptosis of HepG2 cells. nor-NOHA was also able to decrease the expression levels of Arg1 and MMP-2, increase the expression levels of P53 and ECD as well as the production of NO. In addition, nor-NOHA inhibited the invasion and migration of HepG2 cells. These data indicated that nor-NOHA could induce cell apoptosis and inhibit the ability of invasion and migration of HepG2 cells by inhibiting Arg1 [1].
In vivo: Animal study showd that nor-NOHA treatment could fully restore the aortic response to Ach to that of healthy rats. The results also showed that such beneficial effect was mediated by an increase in NOS activity and EDHF and reduced superoxide anion production. In addition, nor-NOHA could decrease IL-6 and VEGF plasma levels in AIA rats. Whereas, the treatment did not modify arthritis severity in AIA rats [2].
Clinical trial: So far, no clinical study has been conducted.
References:[1] Li X, Zhu F, He Y, Luo F. Arginase inhibitor nor-NOHA induces apoptosis and inhibits invasion and migration of HepG2 cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Apr;33(4):477-482.[2] Prati C, Berthelot A, Kantelip B, Wendling D, Demougeot C. Treatment with the arginase inhibitor Nw-hydroxy-nor-L-arginine restores endothelial function in rat adjuvant-induced arthritis. Arthritis Res Ther. 2012 May 30;14(3):R130.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
多谢……
各位大侠：小弟最近做个长片段的CDNA与T载体的连接，片段大小6.4K，载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621)，CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板，操作步骤严格按照说明做的，也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段，与载体连接，结果筛选到的几个克隆，插入的片段仅有2KB左右，奈何？可最近几次再用此反转录试剂盒却不能得到所需片段。在此，小弟想问问大家，你们是否遇到同样的问题，是如何解决的？所用的试剂（载体和酶的选择）和方法是什么，可以告诉后来者，共同进步吧。
