One-Step RT-PCR Kit [Hot-Taq]
- Fast and easy one-tube setup
- Convenient enzyme mix and 2x reaction mix
- Heat-labile UDG system: prevention of carryover contamination
- Convenience and reduced contamination risk
- The highest specificity of PCR amplification
Products
| Cat.No. | Product | Feature | Size |
|---|---|---|---|
| BORTH | One-Step RT-PCR Kit [Hot-Taq] | - | Custom |
| BORTHU | One-Step RT-PCR Kit [Hot-Taq], w/UDG | + UDG SYSTEM* | Custom |
- Description
- Data
- Specification
- Documents
One-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Hot-Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP.
Application
- Viral RNA detection
- Gene-expression analysis
- Molecular Diagnosis
- Endpoint one-step RT-PCR from any RNA transcript
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

