One-Step RT-PCR Kit [Hot-Taq]
- Fast and easy one-tube setup
- Convenient enzyme mix and 2x reaction mix
- Heat-labile UDG system: prevention of carryover contamination
- Convenience and reduced contamination risk
- The highest specificity of PCR amplification
Products
| Cat.No. | Product | Feature | Size |
|---|---|---|---|
| BORTH | One-Step RT-PCR Kit [Hot-Taq] | - | Custom |
| BORTHU | One-Step RT-PCR Kit [Hot-Taq], w/UDG | + UDG SYSTEM* | Custom |
- Description
- Data
- Specification
- Documents
One-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Hot-Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP.
Application
- Viral RNA detection
- Gene-expression analysis
- Molecular Diagnosis
- Endpoint one-step RT-PCR from any RNA transcript
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

