
Description
Formerly DNA Extraction Control 670.
qPCR Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample DNA.Product Highlights
- Simple - streamlined protocol for straightforward validation of DNA extraction and determination of qPCR assay inhibition
- Sensitive - control assay identifies even small effects on DNA extraction and inhibition of amplification
- Optimized - control DNA has a sequence with no known homology to any organism thereby avoiding detection of sample DNA
- Specific - probe-based assay designed specifically for real-time PCR assays
- Flexible - ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum samples
Product Description
A common practice in qPCR is to add a known amount of spiked control DNA after DNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed the qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
The qPCR Extraction Control cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix, which includes primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step. qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
Applications
- Pathogen detection
- Cancer risk assessment
- Gene expression analysis
- Copy number variation (CNV) analysis
- Genotyping
- Viral loading

Application Note
qPCR Extraction Control Redebiomall.com






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没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。
只要是细胞,就得走向衰老,现代研究表明,细胞的衰老和端粒的变短有关系。端粒就是DNA,随着细胞分裂次数的增多,端粒在不断变短,端粒酶就是组织端粒变短的。然而正常细胞中,端粒酶的活性受抑制,端粒酶是怎么染端粒变长的呢?实际上端粒酶是由RNA组成的,可以根据碱基互补配对逆转形成DNA,使得变短的端粒变长。所以说端粒酶可以逆转录形成端粒,使端粒不减短!
我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。

