Description
Product Highlights
- Fast – simple protocol for reliable recovery of DNA and dsRNA in as little as 30 minutes
- Efficient – column-free approach to clean-up promotes recovery of up to 98% of PCR products
- High-performance – extraction of nucleic acid suitable for use in all routine downstream applications
- Convenient – easy-to-use optional pink dye for improved pellet visibility, thereby minimizing sample loss
Product Description
SureClean Plus provides a simple, efficient method for the purification and concentration of DNA or dsRNA from PCR reactions or any enzymatic digests, without the need for hazardous reagents. SureClean Plus removes primers, primer-dimers, dNTPs and proteins.
The protocol allows the precipitation of nucleic acids ≥75 bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns. Samples can be resuspended in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit downstream experimental needs.
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids. The co-precipitant does not interfere with downstream applications.
SureClean Plus has been designed to deliver optimal performance during cloning and after end point PCR.
Applications
- Cloning
- End-point PCR and RT-PCR
- qPCR and RT-qPCR
- Southern Blotting
- Sequencing
- Transfection
- Buffer exchange

Application Note
SureClean Plusebiomall.com
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我在做5RACE,选用TaKaRa公司的RNA反转录酶(RNaseH-),不知道这个酶好不好,有什么需要注意的。
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。

