Description
Product Highlights
- Fast – simple protocol for reliable recovery of DNA and dsRNA in as little as 30 minutes
- Efficient – column-free approach to clean-up promotes recovery of up to 98% of PCR products
- High-performance – extraction of nucleic acid suitable for use in all routine downstream applications
- Convenient – easy-to-use optional pink dye for improved pellet visibility, thereby minimizing sample loss
Product Description
SureClean Plus provides a simple, efficient method for the purification and concentration of DNA or dsRNA from PCR reactions or any enzymatic digests, without the need for hazardous reagents. SureClean Plus removes primers, primer-dimers, dNTPs and proteins.
The protocol allows the precipitation of nucleic acids ≥75 bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns. Samples can be resuspended in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit downstream experimental needs.
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids. The co-precipitant does not interfere with downstream applications.
SureClean Plus has been designed to deliver optimal performance during cloning and after end point PCR.
Applications
- Cloning
- End-point PCR and RT-PCR
- qPCR and RT-qPCR
- Southern Blotting
- Sequencing
- Transfection
- Buffer exchange

Application Note
SureClean Plusebiomall.com
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

