Recombinant Human CUL2/NEDD8/RBX1 Complex Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-421
| Formulation | X mg/ml (XμM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution Calculator
Background: CUL2/NEDD8/RBX1 Complex
Cullin-2 is a core component of multiple cullin-RING based "ECS" (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes. In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to the catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. This complex consists of an N-terminal 10-His tagged Cullin-2 (UniProt Q13617) and untagged RBX1 (P62877). CUL2 is neddylated in this complex.
- Baek K., et al. (2020) Nature 578: 461
- Duda D.M., et al. (2008) Cell 134: 995
- Duda D.M., et al. (2012) Mol. Cell 47: 371
- Goldenberg S.J., et al. (2004) Cell 119: 517
- Zheng N., et al. (2002) Nature 416: 703
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human CUL2/NEDD8/RBX1 Complex Protein, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human CUL2/NEDD8/RBX1 Complex Protein, CF and earn rewards!
Have you used Recombinant Human CUL2/NEDD8/RBX1 Complex Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
多谢……
各位大侠：小弟最近做个长片段的CDNA与T载体的连接，片段大小6.4K，载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621)，CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板，操作步骤严格按照说明做的，也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段，与载体连接，结果筛选到的几个克隆，插入的片段仅有2KB左右，奈何？可最近几次再用此反转录试剂盒却不能得到所需片段。在此，小弟想问问大家，你们是否遇到同样的问题，是如何解决的？所用的试剂（载体和酶的选择）和方法是什么，可以告诉后来者，共同进步吧。
