| Product Name | SMAP - 18NEWRGLRRLGRKIAHGVKKYG - NH2 |
| Size | 1 mg |
| Catalog # | AS-65595 |
| US$ | $147 |
| Purity | ≥95% |
SMAP -18 is 18-residue peptide amide truncated form of SMAP-29. Sheep myeloid antimicrobial peptide-29 (SMAP-29) displays extremely high antimicrobial activity against Pseudomonas strains, other gram-negative bacteria, and multidrug-resistant pathogens1. SMAP-18 displays much higher cell selectivity as compared to parental SMAP-29 because of their decreased hemolytic activity and retained antimicrobial activity2. | |
| Detailed Information | |
| Storage | 20°C |
| References | 1. Saiman, L. et al. Antimicrob. Ag. Chemother. 45, 2838 (2001)2.Jacob B, et al., Amino Acids 46(1),187-98 (2014) |
| Molecular Weight | 2064.62 |
| RGLRRLGRKIAHGVKKYG-NH2 | |
| Sequence(Three-Letter Code) | H - Arg - Gly - Leu - Arg - Arg - Leu - Gly - Arg - Lys - Ile - Ala - His - Gly - Val - Lys - Lys - Tyr - Gly - NH2 |
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

