| Product Name | Ghrelin, humanGS - S(n - octanoyl) - FLSPEHQRVQQRKESKKPPAKLQPR |
| Size | 5 mg |
| Catalog # | AS-24158 |
| US$ | $997 |
| Purity | % Peak Area By HPLC ≥ 95% |
Ghrelin is an appetite stimulating peptide hormone secreted by stomach P/D1-type cells in humans and circulates in the bloodstream during fasting conditions. Enzymatically n-octanoylated Serine3 of this 28-amino acid Ghrelin is the endogenous ligand specific for growth hormone secretagogue receptor 1a (GHSR1a). The GHSR1a receptor appears to be dedicated to Ghrelin and is widely expressed in different tissues. | |
| Detailed Information | |
| Storage | -20°C |
| References | Kojima, M. et al. Nature 402, 656 (1999), doi:10.1038/45230Yang J., et. al. Cell 132, 387 (2008).doi: 10.1016/j.cell.2008.01.017 Kojima, M. et. al. Physiol Rev 85, 495 (2005), doi: 10.1152/physrev.00012.2004 Perello, M. & Dickson, SL. J Neuroendocrinol 27, 424 (2015), doi: 10.1111/jne.12236 |
| Molecular Weight | 3370.9 |
| GS-S(n-octanoyl)-FLSPEHQRVQQRKESKKPPAKLQPR | |
| Sequence(Three-Letter Code) | H - Gly - Ser - [Ser(n - octanoyl)] - Phe - Leu - Ser - Pro - Glu - His - Gln - Arg - Val - Gln - Gln - Arg - Lys - Glu - Ser - Lys - Lys - Pro - Pro - Ala - Lys - Leu - Gln - Pro - Arg - OH |
| Product Citations | Mabudi, H. et al. (2011). The effects of ghrelin on ovary histology in Barbus sharpeyi. J Anim Physiol Anim Nutr 10.1111/j.1439-0396.2010.01089.x. |
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多谢……
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。

