
- Isolate high quality total RNA from hard to extract fatty tissues
- Isolate total RNA, including microRNA (miRNA)
- No phenol or chloroform extractions
- Fast and easy processing using rapid spin-column format
- Genomic DNA removal without the use of nucleases
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Fatty Tissue RNA Purification Kit
This kit was deigned to extract high quality and yield from fatty tissues like brain, breast, adipose tissues, etc. Often tissues that have high fat contents like brain, breast tissues, etc., can lead to low quality and yield RNA with no-optimized kit.
This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA) without the use of inhibitory phenol or chloroform. While the kit is optimized for fatty tissues, it can also be used for most mammalian tissue samples.
The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-qPCR, microarrays, NGS.
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试剂盒里有详细的说明书,告诉你样品需要多少量,每个试剂需要加入多少量,和详细的实验步骤,一般买来就可以用,不用人教。
所以你问一个样需要多少量是没法回答的,测定过程是要加很多种试剂的。
加入荧光标记探针,巧妙地把核算扩增、杂交、光谱分析和实时检测技术结合在一起,借助于荧光信号来检测PCR产物。一方面提高了灵敏度,另一方面还可以做到PCR每循环一次就收集一个数据,建立实时扩增曲线,准确地确定CT值,从而根据CT值确定起始DNA的拷贝数,做到真正意义上的DNA定量。另外由于CT值是一个完全客观的参数,CT值越小,模版DNA的起始拷贝数越小。因此,利用CT值确定DNA拷贝数实时PCR方法比普通终点定量方法更加准确
LOQ-定量限LOD-检测限
至少可以说同一种蛋白定量试剂盒并不适用于所有细胞
蛋白定量试剂盒有分Bradford,BCA,Lowry等,每一种对不同的细胞都有独特的适用范围和灵敏度。所以需要多种蛋白定量试剂盒对比检测才能让结果准确
如果要是直接注射,就不知道了
现在跑tricine-sds-page电泳老是跑不好,
我刚做了bradford定量,
觉得差异与空菌对照还是挺明显的,
但是这个方法只能初略的估计,
还是不能精确定量,
做HPLC我现在还没有条件,
所以我想能不能用BCA或者lowry法先进行定量啊?
这种方法如果做出来结果的话,
能不能发个sci之类的文章啊?
希望高手们指点一下!
谢谢了

