CD244 (2B4, SLAMF4) is a 66 kD member of the CD2/SLAM related receptor family (SRR). It is expressed on NK (3), Tcell subsets, monocytes and eosinophils(4). It is expressed at high levels on Leuikemia initiating cells, and plays a role in their proliferative potential (5). In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer(2). CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.
Antibody ANC244.8 binds to recombinant CD244-muIg (cat #544-020) in EIA, and to cell surface CD244 on human PBL in FACS. ANC244.8 binds to a CD244 epitope that is distinct from the binding sites of clone ANC244.3 (cat #259-020). It blocks binding of clone C1.7.
References:
1) Boles KS, Mathew PA, et al. (1999) Tissue Antigens 54(1): 27-34.
2) Mak A, AM Fairhurst, et al. (2017) Clinical Rheumatology 37(3): 811-816. doi.org/10.1007/s10067-017-3698-2.
3) Vacca P, MC Mingarri, et al. (2006) Blood 108: 4078-4085.
4) Munitz A, Levi-Schaffer F, et al. (2005) J Immunol 174(1): 110-118.
5) Zhang F, Zheng J, et al. (2017) Haematologica Doi: 10.3324/haematol.2016.151555.
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一般来说，试剂盒曲线上的最小浓度是比较准确的，低于这个值因为与曲线是个“S"型结构有关，所以结果是有偏差的，是一种估算。再加上实验误差，所以达不到理想的效果。建议选择试剂盒时，应该看曲线上最小的浓度值，而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想，但在酶活性放大ELISA中，检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子，那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响，往往达不到这个水平(大于104个分子)，但表明ELISA在灵敏度方面的改进潜力是很大的。
