| Artikelnummer | KTC4003-2 |
|---|---|
| Artikelgruppe | Zellfärbekits |
| Artikelbezeichnung | TraKine Mitochondrion Staining Kit (Green Fluorescence) |
| Spezies-Reaktivität | Mammalia |
| Spezifität | Mitochondrien |
| Anwendung | Durchflusszytometrie (Flow Cytometry), Immunfluoreszenz |
| Beschreibung | gleichmäßige Färbung der Mitochondrien von Säugerzelltypen, kann für lebende und fixierte, suspendierte oder adhärente Zellen verwendet werden |
| Inhalt | Assaypuffer (10×), MitoGreen (1000×) |
| Zweckbestimmung | nur für Forschungszwecke |
| Temperatur - Lagerung | gekühlt, komponentenspezifisch |
| Temperatur - Transport | gekühlt |
| Packungsgröße | 500 Tests |
| Hersteller / Marke | Abbkine Scientific |
- KTC4003-2 – Datenblatt
Abbkine Mitochondrion Staining Kits are a set of fluorescence imaging tools to label mitochondria of live cells. This kit uses a proprietary mitochondrial green fluorescent probe (Ex/Em=490/523 nm), which, unlike other probes, appears to localize to mitochondria, much less depending on mitochondrial membrane potential. The dye, a hydrophobic compound, has the added advantage that it is essentially nonfluorescent in aqueous solutions and only becomes fluorescent once it accumulates in the lipid environment of mitochondria, and can stain live cells and formaldehyde-fixed cells, but after permeabilized, the fluorescent signal will be lost. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
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希望知道如何购买的朋友告诉一下,谢谢。
一般来说,试剂盒曲线上的最小浓度是比较准确的,低于这个值因为与曲线是个“S"型结构有关,所以结果是有偏差的,是一种估算。再加上实验误差,所以达不到理想的效果。建议选择试剂盒时,应该看曲线上最小的浓度值,而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想,但在酶活性放大ELISA中,检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子,那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响,往往达不到这个水平(大于104个分子),但表明ELISA在灵敏度方面的改进潜力是很大的。

