![Anaspec/HiLyteᵀᴹ Fluor 594 acid, SE/5 mg/AS-81272-5](images/Anaspec/AS-81272-5.gif)
Product Name | HiLyteᵀᴹFluor 594 acid, SEHiLyte Fluor 594 acid, NHS ester |
Size | 5 mg |
Catalog # | AS-81272-5 |
US$ | $429 |
HiLyte Fluor 594, an alternative to Alexa Fluor 594 and DyLight Fluor 594, has spectral characteristics similar to those of Texas Red. The labeling performance and stability are better than those of Texas Red. It has high extinction coefficient (80,000 M-1cm-1), high fluorescence quantum yield (0.9) and low correction factor (0.17). HiLyte Fluor 594 based Bioconjugates exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.Extinction Coefficient (M-1cm-1): 80,000Fluorescence quantum yield: 0.90Fluorescence Life Time (ns): 4.2 HiLyte Fluor594 acid, SE is an amine reactive reagent for labeling peptides, proteins and antibodies. | |
Detailed Information | ![]() |
Storage | -20°C desiccated and protected from light |
Molecular Weight | 848.94 |
Spectral Properties | Abs/Em = 593/616 nm |
Solvent System | DMF or DMSO |
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希望知道如何购买的朋友告诉一下,谢谢。
一般来说,试剂盒曲线上的最小浓度是比较准确的,低于这个值因为与曲线是个“S"型结构有关,所以结果是有偏差的,是一种估算。再加上实验误差,所以达不到理想的效果。建议选择试剂盒时,应该看曲线上最小的浓度值,而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想,但在酶活性放大ELISA中,检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子,那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响,往往达不到这个水平(大于104个分子),但表明ELISA在灵敏度方面的改进潜力是很大的。
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品,兔抗睾酮抗体,带碘标记的睾酮,和驴抗兔免疫分离剂。实验过后我查了一下生物化学书,发现睾酮这种激素的化学本质非蛋白质,而是缁醇。这样问题就出来了:据我所知,产生免疫反应的是蛋白质,然而性类固醇激素的化学本质是缁醇。请问各位,缁醇也能产生免疫反应吗?
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