The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.View our videos on protocols, tips, and recycling Monarch.
Advantages:
Elute in as little as 30 μl
Prevent buffer retention and salt carry-over with optimized column design
Reduce hands on time with faster protocols and less spin time
Monitor completion of certain steps using colored buffer system
No need to add RNase before starting
Easily label columns using tab and frosted surfaces
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
No hazardous materials fees
Specifications
Culture Volume:
1-5 ml, not to exceed 15 OD units
Binding Capacity:
up to 20 μg
Plasmid Size:
up to 25 kb
Typical Recovery:
up to 20 μg. Yield depends on plasmid copy number, hoststrain, culture volume, and growth conditions.
Elution Volume:
≥ 30 μl
Purity:
A260/280 and A260/230 ≥ 1.8
Protocol Time:
10½ minutes of spin and incubation time
CompatibleDownstreamApplications:
restriction digestion and other enzymatic manipulations,transformation, transfection, DNA sequencing, PCR, labeling,cell-free protein synthesis, etc.
This product is related to the following categories:
Plasmid Purification Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
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