Deprecated: Required parameter $cat_id follows optional parameter $type in /data/ebiomall/systems/hong.php on line 2088
Deprecated: Required parameter $where follows optional parameter $tree_id in /data/ebiomall/systems/hlb.php on line 3505 +,ProteaseK,serineprotease,peptidebonddigestion,papain,pepsin,protease,nucleicacidpurification,DNAisolation,PCRpurification,RNAisolation,InactivationofRnases,InactivationofDnases,enzymeinactivation蚂蚁淘商城
Highly characterized for more consistent performance, Proteinase K is a subtilisin-relatedserine protease that will hydrolyze a variety ofpeptide bonds. Proteinase K is active in a widerange of temperatures and buffers with optimalactivity between 20 and 60°C and a pH between 7.5and 12.0 (1, 2). Activity is stimulated when up to2% SDS or up to 4 M urea are included in the reaction(3). Calcium is important for thermostabilityof Proteinase K but it is not required for catalysis,therefore Proteinase K is also active in bufferscontaining chelating agents such as EDTA (4).
Lot-to-Lot Variability: Competitor ComparisonActivity measurements from three NEB lots of Proteinase K were compared to threelots from another vendor. Reported activity for all NEB lots is 800 Units/ml ± 10%(indicated as a black line with error bars). Direct measurement of the competitor’smaterial using an assay reported on the vendor’s literature, demonstrated a dramaticover-assessment of all three lots.
Activity Measurements: Competitor ComparisonActivity measurements from a single NEB lot were compared to a single lot from seven other vendors. Proteinase K from NEB is sold at 800 Units/ml ± 10% (indicatedas a black line with error bars), whereas most other vendors sell Proteinase Kas > 800 or > 600 Units/ml. While Proteinase K from some vendors was close tothe stated activity values, others varied significantly from the expected amount.Protein Concentration20 mg/ml, approximately, as determined by UV absorption at 280 nm.
Product Source
Engyodontium album (Tritirachium album)
This product is related to the following categories:
杭州沃森生物技术有限公司在发布的核糖核酸酶A(RNASE1)检测试剂盒ELISA Kit for RNASE1 SEA297Bo供应信息,浏览与核糖核酸酶A(RNASE1)检测试剂盒ELISA Kit for RNASE1 SEA297Bo相关的产品或在搜索更多与核糖核酸酶A(RNASE1)检测试剂盒ELISA Kit for RNASE1 SEA297Bo相关的内容。 查看更多>