TriDye™ 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNAincludes fragments ranging from 0.5-10.0kilobases. The 3.0 kb fragment has increased intensity to serve as a reference band.
Recommended gel percentage range: 0.8-1.2%
Optimum separation on 1%
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>
DNase I 是一种核酸内切酶,降解双链或单链DNA,产生5-磷酸末端的单核苷酸及寡核苷酸,在Mg2+存在时,DNase I独立地作用每条DNA链,切割位点是随机分布,在Mn2+存在下,DNase I 作用于DNA双链的大致同一位置,产生钝末端或具1-2个核苷酸突起的DNA片段。来源:牛胰贮存条件:4℃应用:1、用切口平移法进行放射性标记时,可用DNase I在双链DNA上产生随机切口;2、在进行亚硫酸氢盐介导的诱变前,可用DNase... 查看更多>