TriDye™ Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis.The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
Recommended gel percentage range: 4% TBE agarose, 10% to 20% TBE polyacrylamide
Migration of TriDye dyes during electrophoresis*
Gel
Xylene Cyanol
Bromophenol Blue
Orange G
4% Agarose
150 bp
20bp
5bp
5% Agarose
100bp
15bp
5bp
6% PAGE
400bp
50bp
15bp
10% PAGE
200bp
30bp
10bp
4-20% PAGE
300bp
20bp
10bp
20% PAGE
200bp
20bp
10bp
*These migration rates are approximate and variations may occur based on migration conditions (TAE/TBE buffers, buffer pH, voltage etc.).
TriDye™ Ultra Low Range DNA Ladder visualized on a 4% TBE agarose gel and on a 20% TBE polyacrylamide gel. Mass values are for 0.5 µg/lane. Suggested Load: 5 to 10 µl/lane.
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
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