![Kerafast/Anti-Acetylated Mannan [CCRC-M169, 2C2.D8.E5] Antibody (supernatant)/EGA144/5mL (supernatant)](images/kerafast/2909.jpg)
ThismouseIgMkmonoclonalantibodywasgeneratedagainstglycanandrecognizesgumandtomatoacetylatedmannan.
Highlights:
- Reactswithgumandtomatoacetylatedmannan
- SuitableforELISA,ImmunolabelingandImmunofluorescenceapplications
Mannanisoneofthemajorconstituentgroupsofhemicelluloseinthewallofhigherplants.ItcompriseslinearorbranchedpolymersderivedfromsugarssuchasD-mannose,D-galactose,andD-glucose.
FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.
| ProductType: | Antibody |
| Antigen: | AcetylatedMannan |
| AccessionID: | CCRCM169 |
| Isotype: | IgMk |
| Clonality: | Monoclonal |
| CloneName: | CCRCM169:2C2.D8.E5 |
| Reactivity: | Gumandtomato |
| Immunogen: | Pectin/MeBSA |
| SpeciesImmunized: | Mouse |
| Buffer: | Cellculturesupernatant |
| TestedApplications: | ELISA,Immunolabeling,IF |
| Storage: | <1 month="" at="" 4c,="">1monthat-80C |
| Shipped: | Dryice |
Notes:
Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1?3)(1?4)-ß-glucan.
ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).
- RobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArabidopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.
- DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.
- J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.
- G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.
- G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.
- PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.
- PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
- PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.
- PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.
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