ProductDetails
Source:PolyclonalRabbit
Preparation:ProducedfromseraofrabbitsimmunizedwithhighlypureRecombinantHumanVAP-1.Anti-HumanVAP-1-specificantibodywaspurifiedbyaffinitychromatographyemployinganimmobilizedHumanVAP-1matrix.
Immunogen:E.coli derivedRecombinantHumanVAP-1 (PeproTechcatalog#150-16)
SandwichELISA:TodetectHumanVAP-1bysandwichELISA(using100μl/well),aconcentrationof0.5-2.0μg/mlofthisantibodyisrequired.Thisantigenaffinitypurifiedantibody,inconjunctionwithPeproTech’sBiotinylatedRabbitAnti-HumanVAP-1(500-P326BT)asadetectionantibody,allowsthedetectionofatleast2000-4000pg/mlofRecombinantHumanVAP-1.

WesternBlot:TodetectHumanVAP-1byWesternBlotanalysisthisantibodycanbeusedataconcentrationof0.1-0.2μg/ml.WhenusedinconjunctionwithcompatIBLesecondaryreagents,thedetectionlimitforRecombinantHumanVAP-1is1.5-3.0ng/lane,undereitherreducingornon-reducingconditions.


Note:
Additionalapplicationstestedonalot-to-lotbasis.Please contact TechnicalSupportformoreinformation.


CountryOfOrigin: USA
Notforhumanuse.