| Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| Phospho-Akt (Ser473) Monoclonal Antibody | E-AB-21336 | 3B3 | IgG | WB,IHC-p* | Human | 20μL, 60μL, 120μL, 200μL |
Description
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Spec Sheet E-AB-21336 (PDF File)
| Synonyms | AKT1 |
| Swiss-Prot | P31749 |
| Concentration | 1mg/mL |
| Host | Mouse |
| Immunogen | Synthetic Peptide of Phospho-Akt (Ser473) |
| Purification Method | Protein A purification |
| Formulation | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Storage | Store at -20?. Avoid freeze / thaw cycles. |
| Dilution | WB 1:1000-2000, IHC 1:100-200 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
ebiomall.com
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