| ProductName | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| Flag-TagMonoclonalAntibody | E-AB-20028 | 9K2 | IgG | WB* | Flag-Tag | 30μL,60μL,120μL,200μL |
Description
ProteintagsareproteinorpeptidesequenceslocatedeitherontheC-orN-terminalofthetargetprotein,whichfacilitatesoneorseveralofthefollowingcharacteristics:solubility,detection,purification,localizationandexpression.TheDYKDDDDK(FLAG)peptidehasbeenusedextensivelyasageneraltaginexpressionvectors.Thispeptidecanbeexpressedanddetectedwiththeproteinofinterestasanamino-terminalorcarboxy-terminalfusion.N-terminalFLAGvectorsprovideanEkcleavagesiteforremovalofthefusiontag.TheFLAGpeptideislikelytobelocatedonthesurfaceofafusionproteinbecauseofitshydrophilicnature.Asaresult,theFLAGpeptideismorelikelytobeaccessIBLetoantibodies.
SpecSheetE-AB-20028(PDFFile)
| Synonyms | Anti-Flag-Tag |
| Concentration | 1mg/mL |
| Host | Mouse |
| Immunogen | SyntheticPeptide |
| PurificationMethod | Antigenaffinitypurification |
| Formulation | PBSwith0.02%sodiumazide,50%glycerol,PH7.4 |
| Storage | Storeat-20?.Avoidfreeze/thawcycles. |
| Dilution | WB1:500-1:2000 |
Cautionmustbetakentoavoidcontactwithskinoreyes.Insuchacase,rinsethoroughlyatoncewithwater.Donotingest,inhale,orswallow.Seekmedicalattentionimmediately.Wearappropriateprotectiveclothingsuchaslaboratoryoveralls,safetyglassesandgloves.Itisstronglyadvisedthatthisproductshouldbehandledbypeoplewhohavebeenwelltrainedinlaboratorytechniquesandthatitishandledwithcarepursuanttotheprinciplesofgoodlaboratorypractice.Allchemicalsaredeemedpotentiallyharmful.Thevialispronetofallover.Usecaution,especiallywhenthelidisoff.
FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.
*RemarkIcon:
WB=WesternBlotting,IP=Immunoprecipitation,IF=Immunofluorescence,IHC=Immunohistochemistory,IHC-p=ImmunohistochemistoryParaffin,FCM=FlowCytometry,CH=ChIPAssay
Manufacturedby:Elabscience
ebiomall.com
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