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背景资料ProfosEndoTrapblueassay（endotoxinremoval）:TheProfosEndoTrapblueAssayisbasedonaffinitychromatographywithaworkingtimeof~30minutes。Sampleisappliedtothecolumnwithequilibrationbuffer。Fractionsarecollected。Endotoxinremovalrateis>95%percycle。SamplesaremeasuredwithHycultBiotechLALAssay。HycultBiotechMini-LALassay（endotoxindetection）:TheLALassayisanenzyme-basedassaywithaworkingtimeof~45minutes。SamplesandstandardsareincubatedwithLALreagent。Theenzymaticreaction，triggeredbyendotoxin，willcauseayellowcolortodevelopuponcleavageofthechromophore，p-nitroaniline。Theenzymaticreactionisstoppedbytheadditionofaceticacid。Theabsorbanceat405nmismeasuredwithaspectrophotometer。Astandardcurveisobtainedbyplottingtheabsorbance（linear）versusthecorrespondingconcentrationsoftheE。colistandards（log）。Theendotoxinconcentrationofsamples，whicharerunconcurrentlywiththestandards，canbedeterminedfromthestandardcurve。

产品描述Bacterialendotoxin，lipopolysaccharide（LPS），isafever-producingby-productofgram-negativebacteriacommonlyknownaspyrogen。LPSconsistsofapolysaccharide，acoreoligosaccharideandlipidA，whichisresponsIBLeforthetoxiceffects。Inhuman，lipopolysaccharidebindingprotein（LBP）playsacentralroleinresponsetoLPSintheactivationpathwayandintheneutralizationofLPS。Removalofendotoxinisoneofthemostdifficultdownstreamprocessesduringproteinpurification。EndoTrapblue，aspartoftheEndoClearkit，isdevelopedtomeetthemostchallengingrequirementsofendotoxinremoval:thehighestendotoxinremovalcapacitycombinedwithexcellentrecoveryrates。EndoTrapblueisbasedonaffinitychromatographyforcolumnorbatchmode。TheworkingrangeofEndoTrapblueispH4-9anditworkswithanionicstrengthupto600mMNaCl。Proteins，peptides，antibodiesandplasmidDNAcanbeappliedontotheEndoTrapbluecolumn。CustomerspecificbufferscanbeusedwhenenrichedfreshlywithCa2+。BesidestheEndoTrapblue，EndoTrapredisalsoavailable。EndoTrapredhasapHrangeof6-9andiscapabletoworkwithanionicstrengthupto250mM（
荷兰HycultBiotech的宗旨和策略是着重于天然免疫相关疾病的研究，为人类生活质量的提高谋福利。作为免疫学和细胞生物学领域的领导者，特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者，HycultBiotech公司不仅研发这些用于生物科学研究的产品，同时也致力于将这些科研产品转化为临床测试的研究。作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量。