Biological Activity
Potent inhibitor of AMP-activated protein kinase (AMPK) (Ki = 109 nM). Displays no significant activity on several structurally related kinases including ZAPK, SYK, PKCθ, PKA and JAK3. Inhibits AMPK activation induced by AICAR (Cat. No. 2840) and metformin (Cat. No. 2864). Also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6). Promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro. Promotes neural differentiation of hPSCs as part of a chemical cocktail. Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition.Technical Data
The technical data provided above is for guidance only.For batch specific data refer to the Certificate of Analysis.Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
- Role of AMP-activated protein kinase in mechanism of MetF. action.Zhou et al.J.Clin.Invest., 2001;108:1167
- Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism.Yu et al.Nat.Chem.Biol., 2008;4:33
- Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway.Vucicevic et al.Autophagy, 2011;7:40
- Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells.Hao et al.PLoS ONE, 2008;3:2904
- A reliable and efficient protocol for human pluripotent stem cell differentiation into the definitive endoderm based on dispersed single cells.Diekmann et al.Stem Cells Dev., 2015;24:190
- Chemically defined neural conversion of human pluripotent stem cells.Chen et al.Methods Mol.Biol., 2019;1919:59
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Citations for Dorsomorphin dihydrochloride
The citations listed below are publications that use Tocris products.Selected citations for Dorsomorphin dihydrochloride include:
65Citations: Showing 1 - 10
- A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and OrganoidsAuthors: Yang Et al.Cell Stem Cell2020;27:125
- Neurons Derived from Human Induced Pluripotent Stem Cells Integrate into Rat Brain Circuits and Maintain Both Excitatory and Inhibitory Synaptic Activities.Authors: Yin Et al.Eneuro2019;6
- Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia.Authors: Paonessa Et al.Cell Rep2019;26:582
- FMR1 Reactivating Treatments in Fragile X iPSC-Derived Neural Progenitors In Vitro and In Vivo.Authors: Vershkov Et al.Cell Rep2019;26:2531
- Chemically Defined Neural Conversion of Human Pluripotent Stem CellsAuthors: Chen Et al.Methods Mol Biol2019;1919:59
- Inhibition of proliferation and migration of melanoma cells by ketoconazole and Ganoderma immunomodulatory proteins.Authors: Lu Et al.Oncol Lett2019;18:891
- mTORC1/AMPK responses define a core gene set for developmental cell fate switching.Authors: Jaiswal and KimmelBMC Biol2019;17:58
- An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons.Authors: Canfield Et al.Fluids Barriers CNS2019;16:25
- Exosomes regulate neurogenesis and circuit assembly.Authors: Sharma Et al.Proc Natl Acad Sci U S A2019;116:16086
- A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.Authors: Sheng Et al.Nat Commun2018;9:4047
- Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells.Authors: Caires-J?niorNat Commun2018;9(1):475
- Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.Authors: Kutsche Et al.Cell Syst2018;7:438
- Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria.Authors: Hsu Et al.Elife2018;7
- Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development.Authors: Gibb Et al.Development2018;145
- Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.Authors: Takata Et al.Nat Commun2017;8:1339
- Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop.Authors: Lin Et al.Oncotarget2017;8:110426
- Single-Cell Alternative Splicing Analysis with Expedition Reveals Splicing Dynamics during Neuron Differentiation.Authors: Song Et al.Mol Cell2017;67:148
- DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy.Authors: Nguyen Et al.Dev Cell2017;42:9
- Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK.Authors: Dai Et al.Autophagy2017;13:1435
- Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.Authors: Morizane and BonventreNat Protoc2017;12:195
- Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analysesAuthors: Kapeli Et al.Nature Communications2016;7:12143
- LKB1 loss links serine metabolism to DNA methylation and tumorigenesis.Authors: Kottakis Et al.Nature2016;539:390
- Effects of novel muscarinic M3 receptor ligand C1213 in pulmonary arterial hypertension models.Authors: AhmedPhysiological Reports2016;4:e13069
- Ethanol extract of Kalopanax septemlobus leaf induces caspase-dependent apoptosis associated with activation of AMPK in human hepatocellular carcinoma cells.Authors: Park Et al.Int J Oncol2016;48:261
- Effects of thrombin on Ins signalling and glucose uptake in cultured human myotubes.Authors: Al-Bayati Et al.J Diabetes Complications2016;30:1209
- Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy.Authors: Puente Et al.J Biol Chem2016;291:6026
- Cockayne syndrome-derived neurons display reduced synapse density and altered neural network synchrony.Authors: Vessoni Et al.Hum Mol Genet2016;25:1271
- Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal CellsAuthors: Mou Et al.Cell: Stem Cell2016;19:217
- Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior.Authors: Nott Et al.Nat Neurosci2016;19:1497
- Adenosine monophosphate-activated protein kinase activation mediates the leptin-induced attenuation of cognitive impairment in a streptozotocin-induced rat model.Authors: Zhu Et al.Nature2015;9:1998
- A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors.Authors: Rempel Et al.Arch Toxicol2015;89:1599
- Rapid and robust generation of long-term self-renewing human neural stem cells with the ability to generate mature astroglia.Authors: Palm Et al.Int J Mol Sci2015;5:16321
- Transcription factor binding dynamics during human ES cell differentiation.Authors: Tsankov Et al.Biomed Res Int2015;518:344
- Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.Authors: Liao Et al.Exp Ther Med2015;47:469
- Developmental regulation of tau splicing is disrupted in stem cell-derived neurons from frontotemporal dementia patients with the 10 + 16 splice-site mutation in MAPT.Authors: Sposito Et al.Sci Rep2015;24:5260
- Comparative Study on the Cytoprotective Effects of Activated Protein C Treatment in Nonsteatotic and Steatotic Livers under Ischemia-Reperfusion Injury.Authors: Matsuda Et al.BMC Neurosci2015;2015:635041
- Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy.Authors: Khan Et al.J Pharmacol Exp Ther2015;16:42
- Chemical constituents analysis and antidiabetic activity validation of four fern species from Taiwan.Authors: Chen Et al.Exp Neurol2015;16:2497
- A transient wave of BMP signaling in the retina is necessary for Müller glial differentiation.Authors: Ueki Et al.Development2015;142:533
- The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML.Authors: Obba Et al.Nat Protoc2015;11:1114
- The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.Authors: Huang Et al.Nat Genet2015;10:413
- Bmp suppression in mangrove killifish embryos causes a split in the body axis.Authors: Mourabit Et al.PLoS One2014;9:e84786
- BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.Authors: Graham Et al.Nat Neurosci2014;5:5667
- Co-operative Bmp- and Fgf-signaling inputs convert skin wound healing to limb formation in urodele amphibians.Authors: Makanae Et al.Dev Biol2014;396:57
- Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.Authors: Huang Et al.Nat Biotechnol2014;32:84
- Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development.Authors: Holtzhausen Et al.FASEB J2014;28:1248
- Adenosine inhibits tumor cell invasion via receptor-independent mechanisms.Authors: Virtanen Et al.Mol Cancer Res2014;12:1863
- Bmp and Shh signaling mediate the expression of satb2 in the pharyngeal arches.Authors: Sheehan-Rooney Et al.PLoS One2013;8:e59533
- Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.Authors: Reinhardt Et al.PLoS One2013;8:e59252
- EGF stimulates Müller glial proliferation via a BMP-dependent mechanism.Authors: Ueki and RehGlia2013;61:778
- Moderate excess of pyruvate augments osteoclastogenesis.Authors: Fong Et al.Biol Open2013;2:387
- Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells: a potential role for reducing lipid accumulation.Authors: Kim Et al.J Biol Chem2013;288:22693
- Distinct energy requirements for human memory CD4 T-cell homeostatic functions.Authors: Taub Et al.Stem Cells Dev2013;27:342
- Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.Authors: Pol Et al.Lab Chip2013;247:694
- Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.Authors: Brafman Et al.Cell Metab2013;20:369
- Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments.Authors: Li Et al.Nat Commun2013;13:1969
- Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma.Authors: Knelson Et al.J Clin Invest2013;123:4786
- BML-275, an AMPK inhibitor, induces DNA damage, G2/M arrest and apoptosis in human pancreatic cancer cells.Authors: Duong Et al.Int J Oncol2012;41:2227
- LirGlu., a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage.Authors: Hou Et al.J Cereb Blood Flow Metab2012;32:2201
- Engineering artificial signaling centers to polarize embryoid body differentiation.Authors: Petersen Et al.Br J Pharmacol2012;21:647
- Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses.Authors: Shi Et al.FASEB J2012;15:477
- Estrogen inhibits NPY secretion through membrane-associated estrogen receptor (ER)-α in clonal, immortalized hypothalamic neurons.Authors: Dhillon and BelshamInt J Obes (Lond)2011;35:198
- 5-Aminoimidazole-4-carboxyamide-ribonucleoside (AICAR)-stimulated hepatic expression of Cyp4a10, Cyp4a14, Cyp4a31, and other peroxisome proliferator-activated receptor α-responsive mouse genes is AICAR 5'-monophosphate-dependent and AMP-activatedAuthors: Bumpus and JohnsonHum Mol Genet2011;339:886
- Orexin is required for brown adipose tissue development, differentiation, and function.Authors: Sellayah Et al.Autophagy2011;14:478
- Smarcd3b and Gata5 promote a cardiac progenitor fate in the zebrafish embryo.Authors: Lou Et al.Development2011;138:3113
FAQs
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View all Small Molecule FAQsVersaClone cDNA Plasmids
Human AMPK alpha 2 (NP_006243) VersaClone cDNA
Human AMPK alpha 1 (NP_006242) VersaClone cDNA
Human AMPK beta 1 (NP_006244) VersaClone cDNA
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5 μ Dorsomorphin
2 μM dorsomorphin
On acute brain slices, to investigate the role of AMP kinase. The effect was minimal but close to what was expected.
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蚂蚁淘是生物试剂电商平台， 主营：海外试剂抗体，进口实验仪器，海外诊断原料等生物医学科研用品进口。我司希望借助互联网平等而开放的力量，矢志实现生命科学领域全球的信息同步与标准化，简化科学家的采购流程，节省科学家宝贵时间。如需商业合作，请联系info@ebiomall.com 。
1. 包被：用0.05M PH9，碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1～10μg／ml，在每个聚苯乙烯板的反应孔中加0.1ml，4℃过夜。次日，弃去孔内溶液，用洗涤缓冲液洗3次，每次3分钟。
2. 加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃孵育1小时。然后洗涤。（同时做空白孔，阴性对照孔及阳性对照孔）。
3. 加酶标抗体：于各反应孔中，加入新鲜稀释的酶标抗体（经滴定后的稀释度）0.1ml，37℃孵育0.5～1小时，洗涤。
4. 加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃10～30分钟。
5. 终止反应：于各反应孔中加入2M硫酸0.05ml。
6. 结果判定：可于白色背景上，直接用肉眼观察结果：反应孔内颜色越深，阳性程度越强，阴性反应为无色或极浅，依据所呈颜色的深浅，以“+”、“-”号表示。也可测OD值：在ELISA检测仪上，于450nm（若以ABTS显色，则410nm）处，以空白对照孔调零后测各孔OD值，若大于规定的阴性对照OD值的2.1倍，即为阳性。
方法二：用于检测未知抗体的间接法：
用包被缓冲液将已知抗原稀释至1～10μg／ml，每孔加0.1ml，4℃过夜。次日洗涤3次。加一定稀释的待检样品（未知抗体）0.1ml于上述已包被之反应孔中，置37℃孵育1小时，洗涤。（同时做空白、阴性及阳性孔对照）于反应孔中，加入新鲜稀释的酶标第二抗体（抗抗体）0.1ml，37℃孵育30～60分钟，洗涤，最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
热心细致的服务态度打造温暖亲切的购物氛围，有任何问题欢迎随时联系我们！
苏州蚂蚁淘生物科技有限公司
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Abbexa是一家致力于为生命科学研究、药物研发及生物技术公司提供优质抗体、蛋白及检测试剂盒等产品的高科技生物技术公司。基于剑桥大学研发技术优势，Abbexa提供包括一抗、二抗、纯化蛋白、ELISA试剂盒、各种酶类及其它多种试剂与研究工具的大量生命科学研究产品。
目前Abbexa的产品涵盖单克隆抗体、多克隆抗体、纯化蛋白质及生物试剂，此外，为满足研发人员特殊的实验需要，Abbexa还提供抗体/多肽定制服务。严格的质控标准保证了Abbex产品的高性能，如您对产品不满意，Abbexa将直接提供退／换货服务
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PI3-Kinase PI3-激酶
Echelon 可提供PI3-Kinase 实验解决全套方案，从分析服务到实验用抗体、酶和抑制剂等，全面满足科研需求。
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K-1000 |
PI3-K ELISA Kit |
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PI3-K AlphaScreen® |
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Class III PI3-K ELISA Kit |
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Antibodies 抗体 |
Z-R015 |
Anti-huVps34 (Class III PI3-Kinase) Antibody for IP |
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Z-R016 |
Anti-huVps34 (Class III PI3-Kinase) Antibody for WB |
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Inhibitors 抑制剂 |
B-0021 |
Resveratrol (Class IA PI3-K Inhibitor) |
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B-0222 |
Wortmannin (PI3-Kinase Inhibitor) |
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B-0294 |
PI3-Kinase Inhibitor (LY294002) |
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B-0301 |
PI3-Kinase Gamma Inhibitor 1 (AS605240) |
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B-0302 |
PI3-Kinase Gamma Inhibitor 2 (AS604850 |
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B-0303 |
PI3-Kinase Alpha Inhibitor 1 (PI-103) |
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B-0304 |
PI3-Kinase Alpha Inhibitor 2 |
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Enzymes 酶 |
P27-18H |
PI3-K (p-110 alpha/p85 alpha) Active Enzyme |
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P28-10H |
PI3-K (p-110 beta/p85 alpha) Active Enzyme |
Lysolipids 血溶性脂类
Lysolipid Pathway Synthetic Lysophosphatidic Acid (LPA)
◆ Inhibitors 抑制剂系列 ◆ Labeled 标记物系列
◆ Ligands 配体系列 ◆ Ligands 配体系列
◆ Analogs 类似物系列 ◆ Saturated 底物系列
◆ Unsaturated 不饱和系列
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E-4000 |
Autotaxin Enzyme |
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Substrate底物 |
L-2000 |
FS-3 LysoPLD/Autotaxin Substrate |
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Inhibitors抑制剂 |
L-7416 |
LysoPLD/Autotaxin Inhibitor |
Sphingolipids 鞘脂类
Synthetic Sphingolipids
◆ Sphingosine 鞘氨醇 ◆ Ceramide 神经酰胺
◆ Sphingosine 1 Phosphate (S1P ) 鞘氨醇磷酸酯 ◆ Sphingomyelin 鞘磷脂
◆ FTY720P
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Kits 试剂盒 |
K-1900 |
Sphingosine 1 Phosphate ELISA Kit (S1P) |
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K-3500 |
Sphingosine Kinase 1 Activity Assay |
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K-1800 |
Sphingomyelinase Assay Kit |
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K-3200 |
Acid Sphingomyelinase Assay Kit |
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Enzymes 酶 |
E-K068 |
Sphingosine Kinase 1, active |
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E-K069 |
Sphingosine Kinase 2, active |
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Inhibitors 抑制剂 |
B-0024 |
Sphingosine Kinase Inhibitor |
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产品编号 |
特色产品 |
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S-400B |
Sphingomyelin Biotin |
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P-B0SM |
Sphingomyelin Beads |
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S-6100 |
Sphingosine Beads |
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S-6110 |
Sphingosine 1 Phosphate Beads |
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P-B00Ss |
Sphingo Bead Sample Pack |
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G-SM01 |
Sphingomyelin Grip (Equinatoxin) |
Autotaxin(ATX) 自分泌运动因子
Echelon是世界上唯一完整全面提供Autotaxin(ATX) 和Autotaxin(ATX) 相关试剂的供应商。
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K-4100 |
Autotaxin Activity Assay Kit |
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K-4200 |
Autotaxin Inhibitor Screening Kit |
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K-2800B |
Lysophosphatidic Acid Competitive ELISA Kit |
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L-2000 |
FS-3 (LysoPLD / Autotaxin Substrate) |
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L-7416 |
BrP-LPA Autotaxin Inhibitor |
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E-4000 |
Autotaxin Enzyme |
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AG Scientific, Inc.成立于1997年，专注于创新生化制剂以及用于诊断测试开发的定制原材料，以推进临床前研究药物开发。产品涉及抗生素，生物洗涤剂，缓冲液，诊断，酶，医疗设备试剂，蛋白酶K，还原剂，RNase A等。
FD NeuroTech固定的组织将用我们独特的组织防冻溶液（参见Products，目录号PC102）浸润，然后在预冷至-70°C的异戊烷中快速冷冻。冷冻的组织将在切片前储存在-75°C下。
我公司长期供应常规性的生物试剂，耗材批发零售。秉承质量第一 信誉至上 售后完善 以客户标准为原则，为新老客户提供一个值得信任的平台。各种优势产品：life （脂质体2000，3000），Gelred(核酸染料) 。Trizol，TRIzol® LS 。罗氏潮霉素B。 二甲基亚砜(DMSO)。HyClone培养基。gibco 双抗 胰酶 B27 胎牛血清( 16000-044) 胎牛血清( 10099-141) 等。 CST ，ABcam ，santa抗体。日本同仁CCK-8试剂盒。西班牙琼脂糖。另有 sigma，amresco原装试剂大量现货。以上报价均为市场报价，仅供参考！欢迎新老客户咨询选购！18915418616
苏州蚂蚁淘生物科技有限公司成立于2009年，是一家专业提供实验室整体采购方案的专业公司，为广大采购和实验室解决了大量问题，几年来已经成为几乎所有合作伙伴最优秀的合作伙伴，我们一直秉承信誉第一的原则，坚持不卖假货，哪怕利润损失也要为客户提供最好的产品。
我们已经和国外500多个品牌建立了贸易往来，并在国际上享有相当知名度，现已经成为国外近100个品牌中国指定代理，并成为其中国最优秀的代理商。
我们部分客户
1：经销商代表---国药化试，飞世尔，SIGMA，等1000家左右的经销商
2：学校代表---北京大学，清华大学，上海交通大学，复旦大学，浙江大学，武汉大学，厦门大学.....等200所左右大学及100多家附属医院
3：制药公司代表：罗氏，阿斯利康，诺和诺德，和记黄埔，东阳光科......
4：CRO公司代表：睿智化学，药明康德，桑迪亚，美迪西......
我们现有代理品牌如下，还在不断增加中
美国肽公司（APC）是为生命科学界服务的合同制造组织（CMO）。该公司提供用于肽和蛋白质研究的工具，提供广泛的产品和服务，使客户能够发掘从发现研究到下一代治疗药物的生物机制的潜力。
